More on L Proline
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Published date: 2025/03/02
- Location: 580, Taylor, Mississippi, United States
The Psod-tdcB fragment was obtained by overlap-extension PCR25 utilizing the Psod and tdcB fragments. Clones with 7.5 kb deletions in crtYf have been identified by PCR fragment measurement utilizing primers P77/P78. Clones with deletions in crtYf gene were recognized by PCR using primers P75/P76 for 50 bp deletion, P73/P74 for 500 bp, 705 bp deletions and for a tdcB insertion in the crtYf locus. Δcg0716/0723, pXMJ19-Plcpf1-crRNAcrtYf was linearized with XbaI to obtain fragment 7. The ATCC13032 genomic DNA was used as a template to amplify ∼1 kb upstream and downstream homologous arms using primers P63/P64 and P65/P66, wholesale amino acids manufacturer respectively. The C. glutamicum ATCC13032 genome was used as a template to amplify 987 bp upstream and downstream homologous arms utilizing primers P59/P60 and P61/P62, respectively.
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